Structures of ternary complexes of rat DNA polymerase β, a DNA template-primer, and ddCTP
H Pelletier, MR Sawaya, A Kumar, SH Wilson, J Kraut - Science, 1994 - science.org
… Although many auxiliary proteins such as ligases, helicases, … we were unable to see the
Mg2+ ions in electron density … , perhaps the nucleotidyl transfer mechanism that we present …
Mg2+ ions in electron density … , perhaps the nucleotidyl transfer mechanism that we present …
[PDF][PDF] A second divalent metal ion in the group II intron reaction center
PM Gordon, R Fong, JA Piccirilli - Chemistry & biology, 2007 - cell.com
… RNA catalysts that mediate phosphoryl transfer fall into two general classes: (1) large ribozymes
(RNase P and self-splicing introns) that catalyze nucleotidyl transfer … ) that catalyze RNA …
(RNase P and self-splicing introns) that catalyze nucleotidyl transfer … ) that catalyze RNA …
A ribozyme that triphosphorylates RNA 5′-hydroxyl groups
JE Moretti, UF Müller - Nucleic acids research, 2014 - academic.oup.com
… most active clones from the ligase ribozyme-based assay. … The fastest rate is obtained at 400
mM free [Mg2+]. The half-… Mg 2+ ions accelerate the uncatalyzed reaction (63) but no kinetic …
mM free [Mg2+]. The half-… Mg 2+ ions accelerate the uncatalyzed reaction (63) but no kinetic …
Rolling Circle Transcription of Ribozymes Targeted to ras and mdr-1
ET Kool, ROCHESTER UNIV NY - 2000., 2000 - apps.dtic.mil
… site and Kpnl site with T4 DNA ligase. The ligated plasmides were … The stable ribozyme-Mg2+
complex or substrate-Mg2+ … nucleotidyl transfer catalyzed by DNA polymerase I from …
complex or substrate-Mg2+ … nucleotidyl transfer catalyzed by DNA polymerase I from …
Enzymatic properties of rat DNA polymerase β mutants obtained by randomized mutagenesis
A Skandalis, LA Loeb - Nucleic acids research, 2001 - academic.oup.com
… C-terminal domain with nucleotidyl transfer activity and an 8 … gel purified and ligated using
T4 DNA ligase into a ClaI/SphI … The purple spheres represent the Mg2+ ions. This figure was …
T4 DNA ligase into a ClaI/SphI … The purple spheres represent the Mg2+ ions. This figure was …